Construct: shRNA TRCN0000319672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008122.3-2271s21c1
DNA Barcode:
TCCAGAGAAGAACTGATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gjc1 (14615)
Gene Description:
gap junction protein, gamma 1
Transcript:
RefSeq NM_008122.3 (CURRENT)
Match location:
Position 2271 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGAGAAGAACTGATATAT
Hairpin Sequence:
5'-CCGG-TCCAGAGAAGAACTGATATAT-CTCGAG-ATATATCAGTTCTTCTCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGAGAAGAACTGATATATCTCGAGATATATCAGTTCTTCTCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGAGAAGAACTGATATATCTCGAGATATATCAGTTCTTCTCTGGA-3'

Other clones with same target sequence:

(none)