Construct: shRNA TRCN0000319985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177778.4-423s21c1
DNA Barcode:
CAACTACAAGTCCTGGACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc7 (276905)
Gene Description:
armadillo repeat containing 7
Transcript:
RefSeq NM_177778.4 (CURRENT)
Match location:
Position 423 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTACAAGTCCTGGACTTA
Hairpin Sequence:
5'-CCGG-CAACTACAAGTCCTGGACTTA-CTCGAG-TAAGTCCAGGACTTGTAGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTACAAGTCCTGGACTTACTCGAGTAAGTCCAGGACTTGTAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTACAAGTCCTGGACTTACTCGAGTAAGTCCAGGACTTGTAGTTG-3'

Other clones with same target sequence:

  1. TRCN0000180656