Construct: shRNA TRCN0000319995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013534.4-2477s21c1
DNA Barcode:
GCAGAGCAACTGCTTCTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P3h3 (14789)
Gene Description:
prolyl 3-hydroxylase 3
Transcript:
RefSeq NM_013534.4 (CURRENT)
Match location:
Position 2477 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGCAACTGCTTCTAATA
Hairpin Sequence:
5'-CCGG-GCAGAGCAACTGCTTCTAATA-CTCGAG-TATTAGAAGCAGTTGCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGCAACTGCTTCTAATACTCGAGTATTAGAAGCAGTTGCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGCAACTGCTTCTAATACTCGAGTATTAGAAGCAGTTGCTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000178245