Construct: shRNA TRCN0000320090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025740.3-332s21c1
DNA Barcode:
GCTGTTAGAAAGTTACCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap36 (216618)
Gene Description:
cilia and flagella associated protein 36
Transcript:
RefSeq NM_025740.3 (CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTAGAAAGTTACCTCAA
Hairpin Sequence:
5'-CCGG-GCTGTTAGAAAGTTACCTCAA-CTCGAG-TTGAGGTAACTTTCTAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTAGAAAGTTACCTCAACTCGAGTTGAGGTAACTTTCTAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTAGAAAGTTACCTCAACTCGAGTTGAGGTAACTTTCTAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000176928