Transcript: Mouse NM_025740.3

Mus musculus cilia and flagella associated protein 36 (Cfap36), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Cfap36 (216618)
Length:
1523
CDS:
144..1175

Additional Resources:

NCBI RefSeq record:
NM_025740.3
NBCI Gene record:
Cfap36 (216618)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025740.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000177825 CGAATAATCCAGGAGAGGAAT pLKO.1 519 CDS 100% 4.950 6.930 N Cfap36 n/a
2 TRCN0000320091 CGAATAATCCAGGAGAGGAAT pLKO_005 519 CDS 100% 4.950 6.930 N Cfap36 n/a
3 TRCN0000177646 CGATTGCTTGCAGAGAAACTT pLKO.1 1131 CDS 100% 5.625 4.500 N Cfap36 n/a
4 TRCN0000320155 CGATTGCTTGCAGAGAAACTT pLKO_005 1131 CDS 100% 5.625 4.500 N Cfap36 n/a
5 TRCN0000200115 CGTCTGCAGAAATCCAGGTAT pLKO.1 1341 3UTR 100% 4.950 3.960 N Cfap36 n/a
6 TRCN0000198331 GCAAGAGGGAAGGCAATTTAA pLKO.1 1316 3UTR 100% 15.000 10.500 N Cfap36 n/a
7 TRCN0000176808 GCAACTGTGAACTGACTATTA pLKO.1 1279 3UTR 100% 13.200 9.240 N Cfap36 n/a
8 TRCN0000320092 GCAACTGTGAACTGACTATTA pLKO_005 1279 3UTR 100% 13.200 9.240 N Cfap36 n/a
9 TRCN0000200309 GCAAGCATGGAAGGACCAATA pLKO.1 900 CDS 100% 10.800 7.560 N Cfap36 n/a
10 TRCN0000197549 CCTCAGAAGTTAAAGTGCATT pLKO.1 778 CDS 100% 4.950 3.465 N Cfap36 n/a
11 TRCN0000198412 GAGAAAGGATACGAGGACAAA pLKO.1 1004 CDS 100% 4.950 3.465 N Cfap36 n/a
12 TRCN0000176928 GCTGTTAGAAAGTTACCTCAA pLKO.1 332 CDS 100% 4.050 2.835 N Cfap36 n/a
13 TRCN0000320090 GCTGTTAGAAAGTTACCTCAA pLKO_005 332 CDS 100% 4.050 2.835 N Cfap36 n/a
14 TRCN0000137262 GATGTGGTCAGTGACCTTGAA pLKO.1 573 CDS 100% 4.950 2.970 N CFAP36 n/a
15 TRCN0000134138 CAAGCAGAAGAGAGATAAGTT pLKO.1 974 CDS 100% 5.625 3.938 N CFAP36 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025740.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09383 pDONR223 100% 88.4% 90.6% None (many diffs) n/a
2 ccsbBroad304_09383 pLX_304 0% 88.4% 90.6% V5 (many diffs) n/a
3 TRCN0000467508 ACTTGTTACGTTATGCCGCGTAAC pLX_317 38.3% 88.4% 90.6% V5 (many diffs) n/a
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