Construct: shRNA TRCN0000320094

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009840.3-222s21c1
DNA Barcode:
CGGACCAAATGGAATGAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct8 (12469)
Gene Description:
chaperonin containing Tcp1, subunit 8 (theta)
Transcript:
RefSeq NM_009840.3 (CURRENT)
Match location:
Position 222 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320094)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACCAAATGGAATGAATAA
Hairpin Sequence:
5'-CCGG-CGGACCAAATGGAATGAATAA-CTCGAG-TTATTCATTCCATTTGGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACCAAATGGAATGAATAACTCGAGTTATTCATTCCATTTGGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACCAAATGGAATGAATAACTCGAGTTATTCATTCCATTTGGTCCG-3'

Other clones with same target sequence:

  1. TRCN0000120473