Construct: shRNA TRCN0000320111

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010517.3-586s21c1
DNA Barcode:
GACAAGGATGAGAGCGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igfbp4 (16010)
Gene Description:
insulin-like growth factor binding protein 4
Transcript:
RefSeq NM_010517.3 (CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320111)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAGGATGAGAGCGAACAT
Hairpin Sequence:
5'-CCGG-GACAAGGATGAGAGCGAACAT-CTCGAG-ATGTTCGCTCTCATCCTTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAGGATGAGAGCGAACATCTCGAGATGTTCGCTCTCATCCTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAGGATGAGAGCGAACATCTCGAGATGTTCGCTCTCATCCTTGTC-3'

Other clones with same target sequence:

  1. TRCN0000114798