Construct: shRNA TRCN0000320113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010517.3-1921s21c1
DNA Barcode:
CCCTACCCTTATACCTCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igfbp4 (16010)
Gene Description:
insulin-like growth factor binding protein 4
Transcript:
RefSeq NM_010517.3 (CURRENT)
Match location:
Position 1921 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACCCTTATACCTCCTAT
Hairpin Sequence:
5'-CCGG-CCCTACCCTTATACCTCCTAT-CTCGAG-ATAGGAGGTATAAGGGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACCCTTATACCTCCTATCTCGAGATAGGAGGTATAAGGGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACCCTTATACCTCCTATCTCGAGATAGGAGGTATAAGGGTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000114796