Construct: shRNA TRCN0000320337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021345.2-1002s21c1
DNA Barcode:
GCTCCGTTACACTATGTGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hacd3 (57874)
Gene Description:
3-hydroxyacyl-CoA dehydratase 3
Transcript:
RefSeq NM_021345.2 (CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGTTACACTATGTGGAT
Hairpin Sequence:
5'-CCGG-GCTCCGTTACACTATGTGGAT-CTCGAG-ATCCACATAGTGTAACGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGTTACACTATGTGGATCTCGAGATCCACATAGTGTAACGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGTTACACTATGTGGATCTCGAGATCCACATAGTGTAACGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000030059