Construct: shRNA TRCN0000321031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009722.3-810s21c1
DNA Barcode:
GCCGTTTGTAATTCTGCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2a2 (11938)
Gene Description:
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Transcript:
RefSeq NM_009722.3 (CURRENT)
Match location:
Position 810 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTTTGTAATTCTGCTTAT
Hairpin Sequence:
5'-CCGG-GCCGTTTGTAATTCTGCTTAT-CTCGAG-ATAAGCAGAATTACAAACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTTTGTAATTCTGCTTATCTCGAGATAAGCAGAATTACAAACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTTTGTAATTCTGCTTATCTCGAGATAAGCAGAATTACAAACGGC-3'

Other clones with same target sequence:

  1. TRCN0000012429