Construct: shRNA TRCN0000321277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133185.2-1010s21c1
DNA Barcode:
CCCTCCTCATTCCCTGTGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rogdi (66049)
Gene Description:
rogdi homolog
Transcript:
RefSeq NM_133185.2 (CURRENT)
Match location:
Position 1010 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCTCATTCCCTGTGGTA
Hairpin Sequence:
5'-CCGG-CCCTCCTCATTCCCTGTGGTA-CTCGAG-TACCACAGGGAATGAGGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCTCATTCCCTGTGGTACTCGAGTACCACAGGGAATGAGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCTCATTCCCTGTGGTACTCGAGTACCACAGGGAATGAGGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000012233