Construct: shRNA TRCN0000321405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008269.1-1031s21c1
DNA Barcode:
GCACGGTTCAATGGTAGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb6 (15414)
Gene Description:
homeobox B6
Transcript:
RefSeq NM_008269.1 (CURRENT)
Match location:
Position 1031 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGGTTCAATGGTAGATTC
Hairpin Sequence:
5'-CCGG-GCACGGTTCAATGGTAGATTC-CTCGAG-GAATCTACCATTGAACCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGGTTCAATGGTAGATTCCTCGAGGAATCTACCATTGAACCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGGTTCAATGGTAGATTCCTCGAGGAATCTACCATTGAACCGTGC-3'

Other clones with same target sequence:

(none)