Construct: shRNA TRCN0000321552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145819.2-543s21c1
DNA Barcode:
GCTACGCCCAGAAGTACATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mzf1 (109889)
Gene Description:
myeloid zinc finger 1
Transcript:
RefSeq NM_145819.2 (CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGCCCAGAAGTACATTC
Hairpin Sequence:
5'-CCGG-GCTACGCCCAGAAGTACATTC-CTCGAG-GAATGTACTTCTGGGCGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGCCCAGAAGTACATTCCTCGAGGAATGTACTTCTGGGCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGCCCAGAAGTACATTCCTCGAGGAATGTACTTCTGGGCGTAGC-3'

Other clones with same target sequence:

(none)