Construct: shRNA TRCN0000321833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009874.3-638s21c1
DNA Barcode:
CGGGCTCCTGAGTTATTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk7 (12572)
Gene Description:
cyclin-dependent kinase 7
Transcript:
RefSeq NM_009874.3 (CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCTCCTGAGTTATTGTTT
Hairpin Sequence:
5'-CCGG-CGGGCTCCTGAGTTATTGTTT-CTCGAG-AAACAATAACTCAGGAGCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCTCCTGAGTTATTGTTTCTCGAGAAACAATAACTCAGGAGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCTCCTGAGTTATTGTTTCTCGAGAAACAATAACTCAGGAGCCCG-3'

Other clones with same target sequence:

  1. TRCN0000023086