Construct: shRNA TRCN0000322090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011161.5-1166s21c1
DNA Barcode:
GCACCCATGAAATTGAGCAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk11 (19094)
Gene Description:
mitogen-activated protein kinase 11
Transcript:
RefSeq NM_011161.5 (CURRENT)
Match location:
Position 1166 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCCATGAAATTGAGCAGT
Hairpin Sequence:
5'-CCGG-GCACCCATGAAATTGAGCAGT-CTCGAG-ACTGCTCAATTTCATGGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCCATGAAATTGAGCAGTCTCGAGACTGCTCAATTTCATGGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCCATGAAATTGAGCAGTCTCGAGACTGCTCAATTTCATGGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000023248