Construct: shRNA TRCN0000322281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178900.4-1974s21c1
DNA Barcode:
CGGCCAATGTCACCTACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkd2 (101540)
Gene Description:
protein kinase D2
Transcript:
RefSeq NM_178900.4 (CURRENT)
Match location:
Position 1974 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCAATGTCACCTACTTTG
Hairpin Sequence:
5'-CCGG-CGGCCAATGTCACCTACTTTG-CTCGAG-CAAAGTAGGTGACATTGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCAATGTCACCTACTTTGCTCGAGCAAAGTAGGTGACATTGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCAATGTCACCTACTTTGCTCGAGCAAAGTAGGTGACATTGGCCG-3'

Other clones with same target sequence:

(none)