Construct: shRNA TRCN0000322346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178900.4-826s21c1
DNA Barcode:
GTACGACAAGATCCTGCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkd2 (101540)
Gene Description:
protein kinase D2
Transcript:
RefSeq NM_178900.4 (CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGACAAGATCCTGCTCTT
Hairpin Sequence:
5'-CCGG-GTACGACAAGATCCTGCTCTT-CTCGAG-AAGAGCAGGATCTTGTCGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGACAAGATCCTGCTCTTCTCGAGAAGAGCAGGATCTTGTCGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGACAAGATCCTGCTCTTCTCGAGAAGAGCAGGATCTTGTCGTAC-3'

Other clones with same target sequence:

  1. TRCN0000024328