Construct: shRNA TRCN0000322373

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181414.5-1979s21c1
DNA Barcode:
CGTCAAGATCAGCTTATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3c3 (225326)
Gene Description:
phosphatidylinositol 3-kinase catalytic subunit type 3
Transcript:
RefSeq NM_181414.5 (CURRENT)
Match location:
Position 1979 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322373)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGATCAGCTTATTCTT
Hairpin Sequence:
5'-CCGG-CGTCAAGATCAGCTTATTCTT-CTCGAG-AAGAATAAGCTGATCTTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGATCAGCTTATTCTTCTCGAGAAGAATAAGCTGATCTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGATCAGCTTATTCTTCTCGAGAAGAATAAGCTGATCTTGACG-3'

Other clones with same target sequence:

  1. TRCN0000025373