Transcript: Mouse NM_181414.5

Mus musculus phosphatidylinositol 3-kinase catalytic subunit type 3 (Pik3c3), mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Pik3c3 (225326)
Length:
3102
CDS:
56..2719

Additional Resources:

NCBI RefSeq record:
NM_181414.5
NBCI Gene record:
Pik3c3 (225326)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_181414.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380379 AGACATGCTCCGACCTCTATG pLKO_005 210 CDS 100% 10.800 15.120 N Pik3c3 n/a
2 TRCN0000025373 CGTCAAGATCAGCTTATTCTT pLKO.1 1979 CDS 100% 5.625 7.875 N Pik3c3 n/a
3 TRCN0000322373 CGTCAAGATCAGCTTATTCTT pLKO_005 1979 CDS 100% 5.625 7.875 N Pik3c3 n/a
4 TRCN0000025372 GCGTTTAGTACAAGATGGAAT pLKO.1 293 CDS 100% 4.950 6.930 N Pik3c3 n/a
5 TRCN0000380036 AGTAGTCGCTCATCTCTAATT pLKO_005 2914 3UTR 100% 13.200 10.560 N Pik3c3 n/a
6 TRCN0000025371 CCTGTTCAAGAGTGCTCTTAT pLKO.1 1885 CDS 100% 13.200 10.560 N Pik3c3 n/a
7 TRCN0000322374 CTTGCATGCCACTGCTTAAAT pLKO_005 2856 3UTR 100% 15.000 10.500 N Pik3c3 n/a
8 TRCN0000350673 GAACTCAACACTGGCTAATTA pLKO_005 1510 CDS 100% 15.000 10.500 N Pik3c3 n/a
9 TRCN0000322313 ACCACAAGAGATCAGCTAAAT pLKO_005 926 CDS 100% 13.200 9.240 N Pik3c3 n/a
10 TRCN0000322375 CCAAGTGGCCTTGACTATATG pLKO_005 367 CDS 100% 13.200 9.240 N Pik3c3 n/a
11 TRCN0000382405 GGAGGCAAATACCCAGTTATT pLKO_005 1937 CDS 100% 13.200 9.240 N Pik3c3 n/a
12 TRCN0000382372 GAACATCCTACAAGGCGTTTA pLKO_005 279 CDS 100% 10.800 7.560 N Pik3c3 n/a
13 TRCN0000025370 CCAAGAAAGATAGTCAAACTT pLKO.1 1308 CDS 100% 5.625 3.938 N Pik3c3 n/a
14 TRCN0000025369 CCATATAGATTTCGGGTATAT pLKO.1 2329 CDS 100% 1.320 0.924 N Pik3c3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181414.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491942 CCTATCCGGCCGAGTTACGAGTCC pLX_317 14.6% 87.8% 97.9% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14758 pDONR223 60.7% 87.4% 21% None (many diffs) n/a
3 ccsbBroad304_14758 pLX_304 0% 87.4% 21% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000475007 AATCTTGGTAACTGTATGAATAAA pLX_317 19% 87.4% 21% V5 (not translated due to prior stop codon) (many diffs) n/a
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