Construct: shRNA TRCN0000323643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008445.2-3440s21c1
DNA Barcode:
GCTTGGTTAGTGAGAAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif3c (16570)
Gene Description:
kinesin family member 3C
Transcript:
RefSeq NM_008445.2 (CURRENT)
Match location:
Position 3440 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGTTAGTGAGAAGAGAT
Hairpin Sequence:
5'-CCGG-GCTTGGTTAGTGAGAAGAGAT-CTCGAG-ATCTCTTCTCACTAACCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGTTAGTGAGAAGAGATCTCGAGATCTCTTCTCACTAACCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGTTAGTGAGAAGAGATCTCGAGATCTCTTCTCACTAACCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000089808