Construct: shRNA TRCN0000323878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009729.3-592s21c1
DNA Barcode:
CCTAATCCTCTCCACAAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v0c (11984)
Gene Description:
ATPase, H+ transporting, lysosomal V0 subunit C
Transcript:
RefSeq NM_009729.3 (CURRENT)
Match location:
Position 592 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000323878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAATCCTCTCCACAAAGTA
Hairpin Sequence:
5'-CCGG-CCTAATCCTCTCCACAAAGTA-CTCGAG-TACTTTGTGGAGAGGATTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAATCCTCTCCACAAAGTACTCGAGTACTTTGTGGAGAGGATTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAATCCTCTCCACAAAGTACTCGAGTACTTTGTGGAGAGGATTAGG-3'

Other clones with same target sequence:

  1. TRCN0000101612