Construct: shRNA TRCN0000324147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011590.2-374s21c1
DNA Barcode:
GCGGCATTCTCCTAGCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timm17a (21854)
Gene Description:
translocase of inner mitochondrial membrane 17a
Transcript:
RefSeq NM_011590.2 (CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCATTCTCCTAGCTTTAA
Hairpin Sequence:
5'-CCGG-GCGGCATTCTCCTAGCTTTAA-CTCGAG-TTAAAGCTAGGAGAATGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCATTCTCCTAGCTTTAACTCGAGTTAAAGCTAGGAGAATGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCATTCTCCTAGCTTTAACTCGAGTTAAAGCTAGGAGAATGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000114222