Construct: shRNA TRCN0000324167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146090.5-280s21c1
DNA Barcode:
GCCACCCTTTGACATAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zadh2 (225791)
Gene Description:
zinc binding alcohol dehydrogenase, domain containing 2
Transcript:
RefSeq NM_146090.5 (CURRENT)
Match location:
Position 280 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCCTTTGACATAGGTTT
Hairpin Sequence:
5'-CCGG-GCCACCCTTTGACATAGGTTT-CTCGAG-AAACCTATGTCAAAGGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCCTTTGACATAGGTTTCTCGAGAAACCTATGTCAAAGGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCCTTTGACATAGGTTTCTCGAGAAACCTATGTCAAAGGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000114304