Construct: shRNA TRCN0000324456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133838.4-1445s21c1
DNA Barcode:
CCGGTGTATGACGAACTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehd4 (98878)
Gene Description:
EH-domain containing 4
Transcript:
RefSeq NM_133838.4 (CURRENT)
Match location:
Position 1445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGTGTATGACGAACTCTTT
Hairpin Sequence:
5'-CCGG-CCGGTGTATGACGAACTCTTT-CTCGAG-AAAGAGTTCGTCATACACCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGTGTATGACGAACTCTTTCTCGAGAAAGAGTTCGTCATACACCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGTGTATGACGAACTCTTTCTCGAGAAAGAGTTCGTCATACACCGG-3'

Other clones with same target sequence:

  1. TRCN0000093580