Construct: shRNA TRCN0000324709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027204.2-575s21c1
DNA Barcode:
CGAGAAGATCAAAGCAGCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrpl12 (56282)
Gene Description:
mitochondrial ribosomal protein L12
Transcript:
RefSeq NM_027204.2 (CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAAGATCAAAGCAGCCTT
Hairpin Sequence:
5'-CCGG-CGAGAAGATCAAAGCAGCCTT-CTCGAG-AAGGCTGCTTTGATCTTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAAGATCAAAGCAGCCTTCTCGAGAAGGCTGCTTTGATCTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAAGATCAAAGCAGCCTTCTCGAGAAGGCTGCTTTGATCTTCTCG-3'

Other clones with same target sequence:

  1. TRCN0000102522