Construct: shRNA TRCN0000324871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025899.2-357s21c1
DNA Barcode:
GCATCTAGTTTGACTACCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uqcrc2 (67003)
Gene Description:
ubiquinol cytochrome c reductase core protein 2
Transcript:
RefSeq NM_025899.2 (CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000324871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTAGTTTGACTACCAAA
Hairpin Sequence:
5'-CCGG-GCATCTAGTTTGACTACCAAA-CTCGAG-TTTGGTAGTCAAACTAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTAGTTTGACTACCAAACTCGAGTTTGGTAGTCAAACTAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTAGTTTGACTACCAAACTCGAGTTTGGTAGTCAAACTAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000031706