Construct: shRNA TRCN0000325064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009632.2-761s21c1
DNA Barcode:
CGGTTACCAGTCTCTCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parp2 (11546)
Gene Description:
poly (ADP-ribose) polymerase family, member 2
Transcript:
RefSeq NM_009632.2 (CURRENT)
Match location:
Position 761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTACCAGTCTCTCAAGAA
Hairpin Sequence:
5'-CCGG-CGGTTACCAGTCTCTCAAGAA-CTCGAG-TTCTTGAGAGACTGGTAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTACCAGTCTCTCAAGAACTCGAGTTCTTGAGAGACTGGTAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTACCAGTCTCTCAAGAACTCGAGTTCTTGAGAGACTGGTAACCG-3'

Other clones with same target sequence:

  1. TRCN0000071217