Construct: shRNA TRCN0000325289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010049.3-1013s21c1
DNA Barcode:
GTTTGAAGTCTACGAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhfr (13361)
Gene Description:
dihydrofolate reductase
Transcript:
RefSeq NM_010049.3 (CURRENT)
Match location:
Position 1013 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGAAGTCTACGAGAAGAA
Hairpin Sequence:
5'-CCGG-GTTTGAAGTCTACGAGAAGAA-CTCGAG-TTCTTCTCGTAGACTTCAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGAAGTCTACGAGAAGAACTCGAGTTCTTCTCGTAGACTTCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGAAGTCTACGAGAAGAACTCGAGTTCTTCTCGTAGACTTCAAAC-3'

Other clones with same target sequence:

  1. TRCN0000042196