Transcript: Mouse NM_010049.3

Mus musculus dihydrofolate reductase (Dhfr), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Dhfr (13361)
Length:
5307
CDS:
477..1040

Additional Resources:

NCBI RefSeq record:
NM_010049.3
NBCI Gene record:
Dhfr (13361)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010049.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042196 GTTTGAAGTCTACGAGAAGAA pLKO.1 1013 CDS 100% 4.950 6.930 N Dhfr n/a
2 TRCN0000325289 GTTTGAAGTCTACGAGAAGAA pLKO_005 1013 CDS 100% 4.950 6.930 N Dhfr n/a
3 TRCN0000042193 GACTTATTGAACAACCGGAAT pLKO.1 772 CDS 100% 4.050 5.670 N Dhfr n/a
4 TRCN0000325292 GACTTATTGAACAACCGGAAT pLKO_005 772 CDS 100% 4.050 5.670 N Dhfr n/a
5 TRCN0000427080 GTAAACAGAATCTGGTGATTA pLKO_005 613 CDS 100% 13.200 9.240 N DHFR n/a
6 TRCN0000039000 CCTGAGAAGAATCGACCTTTA pLKO.1 660 CDS 100% 10.800 7.560 N DHFR n/a
7 TRCN0000161799 CCTGAGAAGAATCGACCTTTA pLKO.1 660 CDS 100% 10.800 7.560 N DHFR2 n/a
8 TRCN0000042194 CTGGTGATTATGGGTAGGAAA pLKO.1 624 CDS 100% 4.950 3.465 N Dhfr n/a
9 TRCN0000325290 CTGGTGATTATGGGTAGGAAA pLKO_005 624 CDS 100% 4.950 3.465 N Dhfr n/a
10 TRCN0000042197 ACTTCCAAAGAATGACCACAA pLKO.1 577 CDS 100% 4.050 2.835 N Dhfr n/a
11 TRCN0000042195 GACAAGGATCATGCAGGAATT pLKO.1 884 CDS 100% 0.000 0.000 N Dhfr n/a
12 TRCN0000325291 GACAAGGATCATGCAGGAATT pLKO_005 884 CDS 100% 0.000 0.000 N Dhfr n/a
13 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2194 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010049.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00443 pDONR223 100% 88.4% 89.8% None (many diffs) n/a
2 ccsbBroad304_00443 pLX_304 0% 88.4% 89.8% V5 (many diffs) n/a
3 TRCN0000478285 GCAGTACTGAGTGTACAAGCGTAG pLX_317 55.7% 88.4% 89.8% V5 (many diffs) n/a
4 ccsbBroadEn_09809 pDONR223 100% 87% 86.6% None (many diffs) n/a
5 ccsbBroad304_09809 pLX_304 0% 87% 86.6% V5 (many diffs) n/a
6 TRCN0000478413 TATGTCCAACATCTCACTTAAACT pLX_317 66.6% 87% 86.6% V5 (many diffs) n/a
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