Construct: shRNA TRCN0000325332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145392.2-894s21c1
DNA Barcode:
CCCATCTTGAACTGGACTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bag2 (213539)
Gene Description:
BCL2-associated athanogene 2
Transcript:
RefSeq NM_145392.2 (CURRENT)
Match location:
Position 894 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCTTGAACTGGACTCTA
Hairpin Sequence:
5'-CCGG-CCCATCTTGAACTGGACTCTA-CTCGAG-TAGAGTCCAGTTCAAGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCTTGAACTGGACTCTACTCGAGTAGAGTCCAGTTCAAGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCTTGAACTGGACTCTACTCGAGTAGAGTCCAGTTCAAGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000115171