Construct: shRNA TRCN0000325462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008640.2-1406s21c1
DNA Barcode:
GCTTACCTAATCAATTGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Laptm4a (17775)
Gene Description:
lysosomal-associated protein transmembrane 4A
Transcript:
RefSeq NM_008640.2 (CURRENT)
Match location:
Position 1406 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTACCTAATCAATTGTGTT
Hairpin Sequence:
5'-CCGG-GCTTACCTAATCAATTGTGTT-CTCGAG-AACACAATTGATTAGGTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTACCTAATCAATTGTGTTCTCGAGAACACAATTGATTAGGTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTACCTAATCAATTGTGTTCTCGAGAACACAATTGATTAGGTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000110862