Construct: shRNA TRCN0000325471
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011920.3-1185s21c1
- DNA Barcode:
- GCCTCGGTATTCCATCTTTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Abcg2 (26357)
- Gene Description:
- ATP-binding cassette, sub-family G (WHITE), member 2
- Transcript:
- RefSeq NM_011920.3 (CURRENT)
- Match location:
- Position 1185 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000325471)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTCGGTATTCCATCTTTAA
- Hairpin Sequence:
-
5'-CCGG-GCCTCGGTATTCCATCTTTAA-CTCGAG-TTAAAGATGGAATACCGAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCGGTATTCCATCTTTAACTCGAGTTAAAGATGGAATACCGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCGGTATTCCATCTTTAACTCGAGTTAAAGATGGAATACCGAGGC-3'
Other clones with same target sequence:
-
TRCN0000110805
