Construct: shRNA TRCN0000325502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011920.3-814s21c1
DNA Barcode:
TCAGGTTATGTGGTTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcg2 (26357)
Gene Description:
ATP-binding cassette, sub-family G (WHITE), member 2
Transcript:
RefSeq NM_011920.3 (CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGGTTATGTGGTTCAAGAT
Hairpin Sequence:
5'-CCGG-TCAGGTTATGTGGTTCAAGAT-CTCGAG-ATCTTGAACCACATAACCTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGGTTATGTGGTTCAAGATCTCGAGATCTTGAACCACATAACCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGGTTATGTGGTTCAAGATCTCGAGATCTTGAACCACATAACCTGA-3'

Other clones with same target sequence:

  1. TRCN0000110809