Construct: shRNA TRCN0000325588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031170.2-1417s21c1
DNA Barcode:
CCCTGGCTTCAGCTACGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krt8 (16691)
Gene Description:
keratin 8
Transcript:
RefSeq NM_031170.2 (CURRENT)
Match location:
Position 1417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGCTTCAGCTACGGAAT
Hairpin Sequence:
5'-CCGG-CCCTGGCTTCAGCTACGGAAT-CTCGAG-ATTCCGTAGCTGAAGCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGCTTCAGCTACGGAATCTCGAGATTCCGTAGCTGAAGCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGCTTCAGCTACGGAATCTCGAGATTCCGTAGCTGAAGCCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000091875