Construct: shRNA TRCN0000325708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020486.2-2142s21c1
DNA Barcode:
GCCTTCCTCCAGGGAATGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcam (57278)
Gene Description:
basal cell adhesion molecule
Transcript:
RefSeq NM_020486.2 (CURRENT)
Match location:
Position 2142 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCCTCCAGGGAATGTAA
Hairpin Sequence:
5'-CCGG-GCCTTCCTCCAGGGAATGTAA-CTCGAG-TTACATTCCCTGGAGGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCCTCCAGGGAATGTAACTCGAGTTACATTCCCTGGAGGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCCTCCAGGGAATGTAACTCGAGTTACATTCCCTGGAGGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000113600