Construct: shRNA TRCN0000326059
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_054070.2-508s21c1
- DNA Barcode:
- GAAACACTTCGTGCAGTATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Afg3l1 (114896)
- Gene Description:
- AFG3-like AAA ATPase 1
- Transcript:
- RefSeq NM_054070.2 (NON-CURRENT)
- Match location:
- Position 508 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000326059)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAACACTTCGTGCAGTATTA
- Hairpin Sequence:
-
5'-CCGG-GAAACACTTCGTGCAGTATTA-CTCGAG-TAATACTGCACGAAGTGTTTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAACACTTCGTGCAGTATTACTCGAGTAATACTGCACGAAGTGTTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAACACTTCGTGCAGTATTACTCGAGTAATACTGCACGAAGTGTTTC-3'
Other clones with same target sequence:
-
TRCN0000031213
