Construct: shRNA TRCN0000326091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011317.3-1596s21c1
DNA Barcode:
CCTCCATTCTTAACTCTGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Khdrbs1 (20218)
Gene Description:
KH domain containing, RNA binding, signal transduction associated 1
Transcript:
RefSeq NM_011317.3 (NON-CURRENT)
Match location:
Position 1596 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCATTCTTAACTCTGCAT
Hairpin Sequence:
5'-CCGG-CCTCCATTCTTAACTCTGCAT-CTCGAG-ATGCAGAGTTAAGAATGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCATTCTTAACTCTGCATCTCGAGATGCAGAGTTAAGAATGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCATTCTTAACTCTGCATCTCGAGATGCAGAGTTAAGAATGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000102320