Construct: shRNA TRCN0000326370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008071.3-1420s21c1
DNA Barcode:
CCCTGATCTAACCGATGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrb3 (14402)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit beta 3
Transcript:
RefSeq NM_008071.3 (CURRENT)
Match location:
Position 1420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATCTAACCGATGTGAA
Hairpin Sequence:
5'-CCGG-CCCTGATCTAACCGATGTGAA-CTCGAG-TTCACATCGGTTAGATCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATCTAACCGATGTGAACTCGAGTTCACATCGGTTAGATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATCTAACCGATGTGAACTCGAGTTCACATCGGTTAGATCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000102846