Construct: shRNA TRCN0000326504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011778.2-1218s21c1
DNA Barcode:
GCGGTCAGGATGCTAATCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro1b (23789)
Gene Description:
coronin, actin binding protein 1B
Transcript:
RefSeq NM_011778.2 (CURRENT)
Match location:
Position 1218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTCAGGATGCTAATCCAA
Hairpin Sequence:
5'-CCGG-GCGGTCAGGATGCTAATCCAA-CTCGAG-TTGGATTAGCATCCTGACCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTCAGGATGCTAATCCAACTCGAGTTGGATTAGCATCCTGACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTCAGGATGCTAATCCAACTCGAGTTGGATTAGCATCCTGACCGC-3'

Other clones with same target sequence:

  1. TRCN0000090025