Construct: shRNA TRCN0000326571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011778.2-953s21c1
DNA Barcode:
CGGTACTTTGAGATCACAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro1b (23789)
Gene Description:
coronin, actin binding protein 1B
Transcript:
RefSeq NM_011778.2 (CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTACTTTGAGATCACAGAT
Hairpin Sequence:
5'-CCGG-CGGTACTTTGAGATCACAGAT-CTCGAG-ATCTGTGATCTCAAAGTACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTACTTTGAGATCACAGATCTCGAGATCTGTGATCTCAAAGTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTACTTTGAGATCACAGATCTCGAGATCTGTGATCTCAAAGTACCG-3'

Other clones with same target sequence:

  1. TRCN0000090027