Construct: shRNA TRCN0000326626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009945.3-305s21c1
DNA Barcode:
GAGGATAATGGGATGCCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cox7a2 (12866)
Gene Description:
cytochrome c oxidase subunit VIIa 2
Transcript:
RefSeq NM_009945.3 (CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGATAATGGGATGCCAGTT
Hairpin Sequence:
5'-CCGG-GAGGATAATGGGATGCCAGTT-CTCGAG-AACTGGCATCCCATTATCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGATAATGGGATGCCAGTTCTCGAGAACTGGCATCCCATTATCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGATAATGGGATGCCAGTTCTCGAGAACTGGCATCCCATTATCCTC-3'

Other clones with same target sequence:

  1. TRCN0000076486