Construct: shRNA TRCN0000327300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027554.2-2476s21c1
DNA Barcode:
GCGACTCAGTTGTGAATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp38 (74841)
Gene Description:
ubiquitin specific peptidase 38
Transcript:
RefSeq NM_027554.2 (CURRENT)
Match location:
Position 2476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACTCAGTTGTGAATCAAA
Hairpin Sequence:
5'-CCGG-GCGACTCAGTTGTGAATCAAA-CTCGAG-TTTGATTCACAACTGAGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACTCAGTTGTGAATCAAACTCGAGTTTGATTCACAACTGAGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACTCAGTTGTGAATCAAACTCGAGTTTGATTCACAACTGAGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000030875