Construct: shRNA TRCN0000327358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053069.5-574s21c1
DNA Barcode:
CCTTGGAACATCACAGTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg5 (11793)
Gene Description:
autophagy related 5
Transcript:
RefSeq NM_053069.5 (NON-CURRENT)
Match location:
Position 574 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGAACATCACAGTACAT
Hairpin Sequence:
5'-CCGG-CCTTGGAACATCACAGTACAT-CTCGAG-ATGTACTGTGATGTTCCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGAACATCACAGTACATCTCGAGATGTACTGTGATGTTCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGAACATCACAGTACATCTCGAGATGTACTGTGATGTTCCAAGG-3'

Other clones with same target sequence:

  1. TRCN0000099431