Construct: shRNA TRCN0000099431
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_053069.2-576s1c1
- DNA Barcode:
- CCTTGGAACATCACAGTACAT
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Atg5 (11793)
- Gene Description:
- autophagy related 5
- Transcript:
- RefSeq NM_053069.2 (NON-CURRENT)
- Match location:
- Position 576 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000099431)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCTTGGAACATCACAGTACAT
- Hairpin Sequence:
- 5'-CCGG-CCTTGGAACATCACAGTACAT-CTCGAG-ATGTACTGTGATGTTCCAAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTTGGAACATCACAGTACATCTCGAGATGTACTGTGATGTTCCAAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTTGGAACATCACAGTACATCTCGAGATGTACTGTGATGTTCCAAGG-3'