Construct: shRNA TRCN0000327376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016717.3-211s21c1
DNA Barcode:
CTCCTATGTGTCAGGTAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scly (50880)
Gene Description:
selenocysteine lyase
Transcript:
RefSeq NM_016717.3 (CURRENT)
Match location:
Position 211 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTATGTGTCAGGTAGGAA
Hairpin Sequence:
5'-CCGG-CTCCTATGTGTCAGGTAGGAA-CTCGAG-TTCCTACCTGACACATAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTATGTGTCAGGTAGGAACTCGAGTTCCTACCTGACACATAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTATGTGTCAGGTAGGAACTCGAGTTCCTACCTGACACATAGGAG-3'

Other clones with same target sequence:

  1. TRCN0000099414