Construct: shRNA TRCN0000327490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025379.2-219s21c1
DNA Barcode:
GCACCTAGTTTCCATGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cox7b (66142)
Gene Description:
cytochrome c oxidase subunit VIIb
Transcript:
RefSeq NM_025379.2 (CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTAGTTTCCATGACAAA
Hairpin Sequence:
5'-CCGG-GCACCTAGTTTCCATGACAAA-CTCGAG-TTTGTCATGGAAACTAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTAGTTTCCATGACAAACTCGAGTTTGTCATGGAAACTAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTAGTTTCCATGACAAACTCGAGTTTGTCATGGAAACTAGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000076537