Construct: shRNA TRCN0000327619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144829.1-796s21c1
DNA Barcode:
GAGCGAAGTCATGGAAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aarsd1 (69684)
Gene Description:
alanyl-tRNA synthetase domain containing 1
Transcript:
RefSeq NM_144829.1 (CURRENT)
Match location:
Position 796 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGAAGTCATGGAAGTGAA
Hairpin Sequence:
5'-CCGG-GAGCGAAGTCATGGAAGTGAA-CTCGAG-TTCACTTCCATGACTTCGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGAAGTCATGGAAGTGAACTCGAGTTCACTTCCATGACTTCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGAAGTCATGGAAGTGAACTCGAGTTCACTTCCATGACTTCGCTC-3'

Other clones with same target sequence:

  1. TRCN0000102507