Construct: shRNA TRCN0000328038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028077.2-508s21c1
DNA Barcode:
CAAGTGGCCTAAAGCAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1810055G02Rik (72056)
Gene Description:
RIKEN cDNA 1810055G02 gene
Transcript:
RefSeq NM_028077.2 (CURRENT)
Match location:
Position 508 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTGGCCTAAAGCAGTAAA
Hairpin Sequence:
5'-CCGG-CAAGTGGCCTAAAGCAGTAAA-CTCGAG-TTTACTGCTTTAGGCCACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTGGCCTAAAGCAGTAAACTCGAGTTTACTGCTTTAGGCCACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTGGCCTAAAGCAGTAAACTCGAGTTTACTGCTTTAGGCCACTTG-3'

Other clones with same target sequence:

  1. TRCN0000173164