Construct: shRNA TRCN0000328157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029271.2-602s21c1
DNA Barcode:
GATAATTTCATAGTTAGGCAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrpl32 (75398)
Gene Description:
mitochondrial ribosomal protein L32
Transcript:
RefSeq NM_029271.2 (CURRENT)
Match location:
Position 602 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAATTTCATAGTTAGGCAC
Hairpin Sequence:
5'-CCGG-GATAATTTCATAGTTAGGCAC-CTCGAG-GTGCCTAACTATGAAATTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAATTTCATAGTTAGGCACCTCGAGGTGCCTAACTATGAAATTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAATTTCATAGTTAGGCACCTCGAGGTGCCTAACTATGAAATTATC-3'

Other clones with same target sequence:

  1. TRCN0000191547