Construct: shRNA TRCN0000328187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025577.2-401s21c1
DNA Barcode:
TGTCAGAACAGGACCCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2810428I15Rik (66462)
Gene Description:
RIKEN cDNA 2810428I15 gene
Transcript:
RefSeq NM_025577.2 (CURRENT)
Match location:
Position 401 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCAGAACAGGACCCTGAAA
Hairpin Sequence:
5'-CCGG-TGTCAGAACAGGACCCTGAAA-CTCGAG-TTTCAGGGTCCTGTTCTGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCAGAACAGGACCCTGAAACTCGAGTTTCAGGGTCCTGTTCTGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCAGAACAGGACCCTGAAACTCGAGTTTCAGGGTCCTGTTCTGACA-3'

Other clones with same target sequence:

(none)